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Preventing and troubleshooting artefacts in saturation labelled fluorescence 2‐D difference gel electrophoresis (saturation DiGE)
Author(s) -
McNamara Laura E.,
Kantawong Fahsai A.,
Dalby Matthew J.,
Riehle Mathis O.,
Burchmore Richard
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201100135
Subject(s) - saturation (graph theory) , difference gel electrophoresis , troubleshooting , workflow , fluorescence , chemistry , chromatography , biology , computer science , proteomics , optics , biochemistry , physics , mathematics , database , combinatorics , gene , operating system
Saturation DiGE is a powerful but challenging technique for the characterisation of changes in protein expression between two or more scarce samples. In this paper, measures to prevent and troubleshoot artefacts in the saturation DiGE workflow are discussed, with illustration of some examples as they may be encountered in gel images or analysis.