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Biological versus technical variability in 2‐D DIGE experiments with environmental bacteria
Author(s) -
Zech Hajo,
Echtermeyer Christoph,
Wöhlbrand Lars,
Blasius Bernd,
Rabus Ralf
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201100071
Subject(s) - replicate , coefficient of variation , difference gel electrophoresis , multivariate statistics , proteome , biology , abundance (ecology) , principal component analysis , biological system , bacteria , proteomics , statistics , ecology , mathematics , bioinformatics , biochemistry , genetics , gene
Two‐dimensional difference gel electrophoresis (2‐D DIGE) allows for reliable quantification of global protein abundance changes. The threshold of significance for protein abundance changes depends on the experimental variation (biological and technical). This study estimates biological, technical and total variation inherent to 2‐D DIGE analysis of environmental bacteria, using the model organisms “ Aromatoleum aromaticum ” EbN1 and Phaeobacter gallaeciensis DSM 17395. Of both bacteria the soluble proteomes were analyzed from replicate cultures. For strains EbN1 and DSM 17395, respectively, CV revealed a total variation of below 19 and 15%, an average technical variation of 12 and 7%, and an average biological variation of 18 and 17%. Multivariate analysis of variance confirmed domination of biological over technical variance to be significant in most cases. To visualize variances, the complex protein data have been plotted with a multidimensional scaling technique. Furthermore, comparison of different treatment groups (different substrate conditions) demonstrated that variability within groups is significantly smaller than differences caused by treatment.