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Sulfur‐ 36 S stable isotope labeling of amino acids for quantification (SULAQ)
Author(s) -
Jehmlich Nico,
Kopinke FrankDieter,
Lenhard Steffi,
Vogt Carsten,
Herbst FlorianAlexander,
Seifert Jana,
Lissner Ulrike,
Völker Uwe,
Schmidt Frank,
von Bergen Martin
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201100057
Subject(s) - pseudomonas putida , chemistry , sulfur , mass spectrometry , amino acid , stable isotope ratio , chromatography , isotopes of carbon , isotope , sulfate , isotopic labeling , biochemistry , organic chemistry , enzyme , physics , quantum mechanics , total organic carbon
We introduce a universal metabolic labeling strategy using elemental heavy 36 Sulfur ( 36 S) called 36 Sulfur stable isotope labeling of amino acids for quantification (SULAQ). In the proof of principle experiment, Pseudomonas putida KT2440 was grown in defined minimal medium with sodium benzoate or sodium succinate as the sole carbon and 32 S‐ or 36 S‐sodium sulfate as the sole sulfur sources. Quantification using mass spectrometry resulted in 562 proteins with 1991 unique peptides. SULAQ technology can be a valuable alternative strategy for the quantitative comparisons in MS‐based proteomics approaches characterizing bacterial and other biological samples in different growth conditions.