Premium
Mass spectrometric immunoassay for quantitative determination of transthyretin and its variants
Author(s) -
Trenchevska Olgica,
Kamcheva Elena,
Nedelkov Dobrin
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201100023
Subject(s) - transthyretin , immunoassay , chemistry , retinol binding protein , human plasma , chromatography , multiplex , biochemistry , biology , retinol , antibody , bioinformatics , endocrinology , genetics , vitamin
Transthyretin (TTR, or prealbumin) is a tetrameric protein found in plasma and cerebrospinal fluid. Its major role is to transport thyroid hormones (thyroxin‐T4) and retinol (through association with retinol‐binding protein). TTR has been studied extensively due to the great number of point mutations that result in sequence heterogeneity. Many of these variants are associated with pathological conditions that result in extracellular deposition of amyloid fibers in tissues. In this work, we have developed a rapid mass spectrometric immunoassay for determination and quantification of TTR and its variants from human serum and plasma samples. The assay was fully characterized in terms of its precision, linearity and recovery characteristics. The new assay was also compared with a conventional TTR ELISA. Furthermore, we have applied the optimized method to analyze TTR and its modifications in 44 human plasma samples, and in the process optimized a method for TTR proteolytic digestion and identification of point mutations.