In this issue

Just in time for a late summer holiday: Bait and prey for TAPping TAP is a procedure that classically uses in vivo expression of a complex construct to capture cleanly the members of a particular group of networked proteins. Classically, the signal from the test is amplified by in vivo expression of the “bait.” Besides bait, elements of the procedure include “tags” and “prey”. In work on the regulatory protein CARP‐1, Erlbruch et al. made a helpful technical observation: simply adding purified bait protein to the unamplified prey lysate eliminated a number of problems and allowed them greater control of the reaction conditions. They were able to find a number of binding partners for protein kinase A catalytic subunit Cβ1 including CARP‐1, a cell cycle and apoptosis regulatory factor that had not been verified before. pp. 2890–2900Hot heads and cool hand cakes Oryza sativa , in case you don't recognize the name, is breakfast, lunch, and dinner for half the world's population. It's amazing what we still don't know about rice, but there you are. Combine this general dearth of knowledge with the increasing breadth of Mother Nature's mood swings in temperature and rainfall and you have a real challenge if you are trying to raise rice. Gammulla et al. took it upon themselves to explore the effect of temperature changes on the proteome of cultured rice callus. Ranging from 12 to 44°C in four intervals, they used label‐free shotgun proteomic tools to analyze over 1900 proteins that were expressed at one or more of the temperature points. Some 40 of the proteins newly identified were novel stress response elements. A major metabolic change was the use of alternative sucrose pathways at extreme temperatures. pp. 3001–3019Massy platform for parsimonious analysis of proteomic results The accumulation of proteomic data appears to be progressing at an exponential rate. In an attempt to stem the looming flood of inferred peptide–protein connections, Slotta et al. have developed MassSieve, a new Java software package for comparative visualization analysis and parsimony analysis of LC‐MS/MS proteome data – peptide data in particular. Currently the program runs under Windows, Mac OSX, and Linux and accepts experimental data from MascotData, DAT files, OMSSA XML, X!Tandem XML, and other PepXML output. It uses Prefuse for visualization of protein–peptide relationships, ANTLR for peptide set notation language, and GlazedLists for table manipulation and display. Data can be examined quickly at a high level, then just as quickly drilled down through for indepth analysis. Output is in comma separated value format. pp. 3035–3039