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A novel procedure for protein extraction from formalin‐fixed paraffin‐embedded tissues
Author(s) -
RodríguezRigueiro Teresa,
ValladaresAyerbes Manuel,
HazConde Mar,
Blanco Moisés,
Aparicio Guadalupe,
FernándezPuente Patricia,
Blanco Francisco J.,
Lorenzo Mª José,
Aparicio Luis A.,
Figueroa Angélica
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000809
Subject(s) - immunohistochemistry , blot , extraction (chemistry) , antigen retrieval , chromatography , microbiology and biotechnology , chemistry , biology , computational biology , biochemistry , immunology , gene
Most of the archived pathological specimens in hospitals are kept as formalin‐fixed paraffin‐embedded tissues (FFPE) for long‐term preservation. Up to now, these samples are only used for immunohistochemistry in a clinical routine as it is difficult to recover intact protein from these FFPE tissues. Here, we report a novel, short time‐consuming and cost‐effective method to extract full‐length, non‐degraded proteins from FFPE tissues. This procedure is combined with an effective and non‐toxic deparaffinisation process and an extraction method based on antigen‐retrieval, high concentration of SDS and high temperature. We have obtained enough intact protein to be detected by Western blotting analysis. This technique will allow utilising these stored FFPE tissues in several applications for protein analysis helping to advance the translational studies in cancer and other diseases.