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Characterizing the proteome of the human dorsolateral prefrontal cortex by shotgun mass spectrometry
Author(s) -
MartinsdeSouza Daniel,
Guest Paul C.,
Steeb Hannah,
Pietsch Sandra,
Rahmoune Hassan,
Harris Laura W.,
Bahn Sabine
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000718
Subject(s) - proteome , dorsolateral prefrontal cortex , mass spectrometry , shotgun , shotgun proteomics , prefrontal cortex , proteomics , computational biology , chromatography , biology , chemistry , neuroscience , biochemistry , cognition , gene
Abstract The studies of neuropsychiatric disorders would be facilitated by enhanced knowledge of the dorsolateral prefrontal cortex (DLPFC) proteome. To construct a data set of human DLPFC proteins, protein extracts were prepared from 12 postmortem brains focussing on the DLPFC region (Brodmann area 9) and analyzed using a combined gel electrophoresis and shotgun mass spectrometry approach, featuring data‐independent label‐free nanoflow liquid chromatography mass spectrometry (nLC‐MS E ). The detected mass/time features were aligned and annotated using the results from ProteinLynx Global Server. The resulting data set comprised 488 unique and accurately identified proteins, with stringent identification by a minimum of two distinct peptides detected at least in >75% of samples. These proteins were involved predominantly in cytoskeletal architecture, metabolism, transcription/translation, and synaptic function. Combination of this data set with that obtained by our previous characterization of the same brain region results in a total of 755 unique proteins, making this the most comprehensive analysis of this important brain region to date.