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Large‐scale N‐glycoproteome map of rat brain tissue: Simultaneous characterization of insoluble and soluble protein fractions
Author(s) -
Qiao Xiaoqiang,
Tao Dingyin,
Qu Yanyan,
Sun Liangliang,
Gao Liang,
Zhang Xiaodan,
Liang Zhen,
Zhang Lihua,
Zhang Yukui
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000715
Subject(s) - glycoprotein , glycosylation , solubilization , transmembrane protein , chemistry , biochemistry , fraction (chemistry) , tetrafluoroborate , chromatography , ionic liquid , receptor , catalysis
The large‐scale N‐glycosylation analysis is critical for biomedical research, since a variety of diseases are found to be associated with glycoproteins. By a combination of glycoprotein analysis in insoluble protein fraction solubilized with 1% v/v 1‐butyl‐3‐methylimidazolium tetrafluoroborate (BMIM BF 4 ) and those in soluble fraction, a total number of 462 non‐redundant N‐glycoprotein groups, including 316 transmembrane glycoproteins, were successfully identified. Correspondingly, 849 unique N‐glycosites were confidently recognized. The data set could provide a support for the further in‐depth research of brain N‐glycosylation, such as for the discovery of candidate drug targets and biomarkers.