Premium
Genomic and proteomic characterization of Staphylococcus aureus mastitis isolates of bovine origin
Author(s) -
Wolf Carmen,
Kusch Harald,
Monecke Stefan,
Albrecht Dirk,
Holtfreter Silva,
von Eiff Christof,
Petzl Wolfram,
Rainard Pascal,
Bröker Barbara M.,
Engelmann Susanne
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000698
Subject(s) - mastitis , proteases , biology , virulence , staphylococcus aureus , multilocus sequence typing , proteome , microbiology and biotechnology , gene , genetics , genotype , bacteria , enzyme , biochemistry
Staphylococcus aureus colonizes and infects humans as well as animals. In the present study, 17 S. aureus strains isolated from cows suffering from mastitis were characterized. The well‐established multilocus sequence typing (MLST) technique and a diagnostic microarray covering 185 S. aureus virulence and resistance genes were used for genetic and epidemiological analyses. Virulence gene expression studies were performed by analyzing the extracellular protein pattern of each isolate on 2‐D gels. By this way, a pronounced heterogeneity of the extracellular proteome between the bovine isolates has been observed which was attributed to genome plasticity and variation of gene expression. Merely 12 proteins were expressed in at least 80% of the isolates, i.e. Atl, Aur, GlpQ, Hla, LtaS, Nuc, PdhB, SAB0846, SAB2176, SAB0566, SspA, and SspB forming the core exoproteome. Fifteen extracellular proteins were highly variably expressed and only present in less than 20% of the isolates. This includes the serine proteases SplB, C, and F, and the superantigens SEC‐bov, SEL and TSST‐1. Compared to human isolates we identified at least six proteins with significantly different expression frequencies. While SAB0846 was expressed more frequently in bovine isolates, LytM, EbpS, Spa, Geh, and LukL1 were seen less frequently in these isolates.