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Constructing the metabolic and regulatory pathways in germinating rice seeds through proteomic approach
Author(s) -
He Dongli,
Han Chao,
Yao Jialing,
Shen Shihua,
Yang Pingfang
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000598
Subject(s) - germination , biology , metabolic pathway , transcription factor , shotgun proteomics , proteomics , microbiology and biotechnology , gene expression , gene , organism , computational biology , biochemistry , botany , genetics
Construction of metabolic and regulatory pathways from proteomic data can contextualize the large‐scale data within the overall physiological scheme of an organism. It is an efficient way to predict metabolic phenotype or regulatory style. We did protein profiling in the germinating rice seeds through 1‐DE via LC MS/MS proteomic shotgun strategy. In total, 673 proteins were identified, and could be sorted into 14 functional groups. The largest group was metabolism related. The metabolic proteins were integrated into different metabolic pathways to show the style of reserves mobilization and precursor preparation during the germination. Analysis of the regulatory proteins indicated that regulation of redox homeostasis and gene expression also play important roles for the rice seed germination. Although transcription is unnecessary for the germination, it could ensure the rapidity and uniformity of germination. On the contrary, translation with the stored mRNA is required for the germination. This study will help us to further understand the metabolic style, regulation of redox homeostasis, and gene expression during rice seed germination.