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Metaproteome analysis of sewage sludge from membrane bioreactors
Author(s) -
Kuhn Ramona,
Benndorf Dirk,
Rapp Erdmann,
Reichl Udo,
Palese Luigi Leonardo,
Pollice Alfieri
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000590
Subject(s) - membrane bioreactor , activated sludge , bioreactor , chromatography , chemistry , nitrosomonas europaea , bacteria , wastewater , sewage , kjeldahl method , sewage treatment , protein purification , extraction (chemistry) , nitrification , biochemistry , biology , membrane , waste management , organic chemistry , nitrogen , engineering , genetics
Microbial dynamics and enzymatic activities of activated sludge processes are not completely understood yet. A better understanding about the biology is indispensable for further process optimization. Since proteins play a key role as catalysts in sludge processes, a protocol for protein extraction and analysis by 2‐D PAGE was established. It is based on phenol extraction of alkaline extracts and on a subsequent precipitation with ammonium sulphate. 2‐D protein patterns obtained from different sludges collected from membrane bioreactors showed – besides common spots – significant differences. Selected proteins were identified with nano‐HPLC‐ESI‐MS/MS. All membrane biological reactor (MBR) sludge samples investigated in this study contained elastase 3A, which implies that this human serine protease is a significant constituent of municipal wastewater. Although the identification of proteins from ammonia‐oxidizing bacterium Nitrosomonas europaea was expected, the detection of a protein with homology to the marine bacterium Saprospira grandis in MBR1 was surprising.