Premium
Evaluation and optimization of IgY Spin Column technology in the depletion of abundant proteins from human serum
Author(s) -
Corrigan Laura,
Jefferies Caroline,
Clive Lee Thomas,
Daly Jacqueline
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000587
Subject(s) - biomarker , chromatography , chemistry , proteomics , blood proteins , biology , microbiology and biotechnology , biochemistry , gene
Serum depletion strategies are commonly implemented in order to remove abundant proteins, increasing the number of proteins detected in a biomarker study. The IgY spin columns used in this study bind 12 and 14 primate proteins, respectively. 1‐D SDS‐PAGE and 2‐DE revealed a suboptimal performance of the IgY spin columns. However, modification of the manufacturer's protocol, subjecting samples to two rounds of depletion, improved the number of proteins resolved by 2‐DE. With alteration of the manufacturer protocol, the Seppro ® IgY14 spin column can produce depleted serum with an increased number of spots resolved by 2‐DE compared to untreated serum.