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Proteomic analysis of the effects of the immunomodulatory mycotoxin deoxynivalenol
Author(s) -
da Costa André Nogueira,
Mijal Renée S.,
Keen Jeffrey N.,
Findlay John B. C.,
Wild Christopher P.
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000580
Subject(s) - mycotoxin , biology , toxicity , lactate dehydrogenase , triosephosphate isomerase , protein subunit , proteasome , immune system , flow cytometry , microbiology and biotechnology , biochemistry , chemistry , enzyme , immunology , food science , organic chemistry , gene
The mycotoxin deoxynivalenol (DON) contaminates cereals worldwide and is a common contaminant in the Western European diet. At high doses, DON induces acute gastrointestinal toxicity; chronic, low‐dose effects in humans are not well described, but immunotoxicity has been reported. In this study, 2‐DE was used to identify proteomic changes in human B (RPMI1788) and T (JurkatE6.1) lymphocyte cell lines after exposure to minimally toxic concentrations (up to 500 ng/mL) for 24 h. Proteins which changed their abundance post treatment, by a greater than 1.4‐fold change reproducible in three separate experiments consisting of 36 gels in total, are ubiquitin carboxyl‐terminal hydrolase isozyme L3, proteasome subunit β type‐4 and α type‐6, inosine‐5′‐monophosphate dehydrogenase 2, GMP synthase, microtubule‐associated protein RP/EB family member 1 (EB1), RNA polymerases I, II, III subunit ABC1, triosephosphate isomerase and transketolase. Flow cytometry was used to validate changes to protein expression, except for EB1. These findings provide insights as to how low‐dose exposure to DON may affect human immune function and may provide mechanism‐based biomarkers for DON exposure.