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Membrane‐SPINE: An improved method to identify protein–protein interaction partners of membrane proteins in vivo
Author(s) -
Müller Volker S.,
Jungblut Peter R.,
Meyer Thomas F.,
Hunke Sabine
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000558
Subject(s) - membrane protein , membrane , vesicle associated membrane protein 8 , protein–protein interaction , microbiology and biotechnology , in vivo , chemistry , biology , integral membrane protein , biochemistry , biophysics
Membrane proteins are crucial for many essential cellular processes. As membrane proteins function in complexes, methods to detect and to characterize membrane protein–protein interactions are undoubtedly required. Therefore, we developed the “Membrane‐Strep‐tagged protein interaction experiment” (Membrane‐SPINE) that combines the specific purification of a Strep‐tagged membrane protein with the reversible fixation of protein complexes by formaldehyde cross‐linking. In combination with MS analysis, we suggest Membrane‐SPINE as a powerful tool to identify unknown interaction partners of membrane proteins in vivo.