Premium
Direct quantitation of MHC‐bound peptide epitopes by selected reaction monitoring
Author(s) -
Tan Chor Teck,
Croft Nathan P.,
Dudek Nadine L.,
Williamson Nicholas A.,
Purcell Anthony W.
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000531
Subject(s) - epitope , immunogenicity , computational biology , tandem mass spectrometry , epitope mapping , chemistry , mass spectrometry , antigen , peptide , linear epitope , biology , biochemistry , chromatography , immunology
We describe a cell‐free approach that employs selected reaction monitoring (SRM) in tandem mass spectrometry to identify and quantitate T‐cell epitopes. This approach utilises multiple epitope‐specific SRM transitions to identify known T‐cell epitopes and an absolute quantitation (AQUA) peptide strategy to afford AQUA. The advantage of a mass spectrometry‐based approach over more traditional cell‐based assays resides in the robustness and transferability of an SRM approach between laboratories and the ability of this strategy to detect multiple peptides simultaneously without the requirement of epitope‐specific reagents such as T‐cell lines. Thus, the SRM strategy for epitope quantitation will find application in studies of antigen density, the link between epitope abundance and immunogenicity, the dynamic range of epitope presentation and the abundance of T‐cell epitopes in disease.