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Comprehensive two‐dimensional liquid chromatography mass spectrometric profiling of the rat hippocampal proteome
Author(s) -
Yang Xiaoping,
Levin Yishai,
Rahmoune Hassan,
Ma Dan,
Schöffmann Stephanie,
Umrania Yagnesh,
Guest Paul C.,
Bahn Sabine
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000525
Subject(s) - proteome , hippocampal formation , proteomics , chromatography , chemistry , profiling (computer programming) , mass spectrometry , membrane protein , computational biology , biology , biochemistry , membrane , neuroscience , computer science , gene , operating system
In this study, we performed the first high‐throughput and comprehensive proteomic profiling of the rat hippocampal proteome. Using a combination of 2‐D LC‐MS and data analysis with the Rosetta Elucidator ® system, we identified 1340 unique proteins. Functional classification showed that most of these were associated with synaptic function and comprised a high proportion of phosphorylated proteins and analytically challenging classes of membrane proteins such as ion channel receptor subunits.