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A comparison of MS2‐based label‐free quantitative proteomic techniques with regards to accuracy and precision
Author(s) -
Colaert Niklaas,
Vandekerckhove Joël,
Gevaert Kris,
Martens Lennart
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000521
Subject(s) - shotgun proteomics , quantitative proteomics , proteome , shotgun , computer science , label free quantification , proteomics , fragmentation (computing) , data mining , computational biology , bioinformatics , chemistry , biology , biochemistry , gene , operating system
The advent of algorithms for fragmentation spectrum‐based label‐free quantitative proteomics has enabled straightforward quantification of shotgun proteomic experiments. Despite the popularity of these approaches, few studies have been performed to assess their performance. We have therefore profiled the precision and the accuracy of three distinct relative label‐free methods on both the protein and the proteome level. We derived our test data from two well‐characterized publicly available quantitative data sets.