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iTRAQ‐based proteomic study of the effects of microcystin‐LR on medaka fish liver
Author(s) -
Malécot Mélodie,
Marie Arul,
PuiseuxDao Simone,
Edery Marc
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000512
Subject(s) - microcystin lr , hepatotoxin , microcystin , cyanotoxin , biology , cylindrospermopsin , biochemistry , oryzias , cyanobacteria , marine toxin , toxin , proteomics , protein kinase a , kinase , chemistry , bacteria , toxicity , gene , genetics , organic chemistry
Microcystins are cyanotoxins that occur in ground water and thus pose a potential health risk. Microcystin‐LR (microcystin‐leucine‐arginine) is a potent hepatotoxin, and is suspected of being a tumour promoter. Poisoning with this toxin causes several dysfunctions in hepatocytes by inhibiting protein phosphatases 1 and 2A, and notably produces oxidative stress, disrupts the cytoskeleton, and deregulates mitogen‐activated protein kinase pathway. Medaka fish ( Oryzias latipes ) was chosen as a model for studying the effects of this cyanotoxin on liver proteins using a gel‐free approach, iTRAQ. Fish were gavaged with microcystin‐LR. Two hours later, 325 proteins could be identified by Scaffold Q+ and 32 proteins revealed statistically significant variations above a ∣0.2∣ threshold of log 2 ratio by comparison with control. These proteins are mostly involved in the translation and maturation of proteins, lipid metabolism and detoxification. Notably, apolipoproteins are deregulated which indicates a possible alteration of chylomicron‐mediated transport.