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Bacillus subtilis YqjG is required for genetic competence development
Author(s) -
Saller Manfred J.,
Otto Andreas,
BerrelkampLahpor Greetje A.,
Becher Dörte,
Hecker Michael,
Driessen Arnold J. M.
Publication year - 2010
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000435
Subject(s) - bacillus subtilis , proteome , biology , microbiology and biotechnology , transmembrane domain , membrane protein , protein domain , computational biology , function (biology) , biochemistry , gene , genetics , membrane , bacteria
Members of the evolutionary conserved Oxa1/Alb3/YidC family have been shown to play an important role in membrane protein insertion, folding and/or assembly. Bacillus subtilis contains two YidC‐like proteins, denoted as SpoIIIJ and YqjG. SpoIIIJ and YqjG are largely exchangeable, but SpoIIIJ is essential for spore formation and YqjG cannot complement this activity. To elucidate the role of YqjG, we determined the membrane proteome and functional aspects of B. subtilis cells devoid of SpoIIIJ, YqjG or both. The data show that SpoIIIJ and YqjG have complementary functions in membrane protein insertion and assembly. The reduced levels of F 1 F O ATP synthase in cells devoid of both SpoIIIJ and YqjG are due to a defective assembly of the F 1 ‐domain onto the F 0 ‐domain. Importantly, for the first time, a specific function is demonstrated for YqjG in genetic competence development.