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Landscape of the SOX2 protein–protein interactome
Author(s) -
Fang Xuefeng,
Yoon JaeGeun,
Li Lisha,
Tsai Yihsuan S.,
Zheng Shu,
Hood Leroy,
Goodlett David R.,
Foltz Gregory,
Lin Biaoyang
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000419
Subject(s) - interactome , ribonucleoprotein , biology , sox2 , immunoprecipitation , gene , microbiology and biotechnology , rna helicase a , small nuclear ribonucleoprotein , transcription factor , rna splicing , protein–protein interaction , genetics , helicase , rna
SOX2 is a key gene implicated in maintaining the stemness of embryonic and adult stem cells that appears to re‐activate in several human cancers including glioblastoma multiforme. Using immunoprecipitation (IP)/MS/MS, we identified 144 proteins that are putative SOX2 interacting proteins. Of note, SOX2 was found to interact with several heterogeneous nuclear ribonucleoprotein family proteins, including HNRNPA2B1, HNRNPA3, HNRNPC, HNRNPK, HNRNPL, HNRNPM, HNRNPR, HNRNPU, as well as other ribonucleoproteins, DNA repair proteins and helicases. Gene ontology (GO) analysis revealed that the SOX2 interactome was enriched for GO terms GO:0030529 ribonucleoprotein complex and GO:0004386 helicase activity. These findings indicate that SOX2 associates with the heterogeneous nuclear ribonucleoprotein complex, suggesting a possible role for SOX2 in post‐transcriptional regulation in addition to its function as a transcription factor.