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Comprehensive proteomic and transcriptomic characterization of hepatic expression signatures affected in p14 liver conditional knockout mice
Author(s) -
Prokudin Ivan,
Stasyk Taras,
Rainer Johannes,
Bonn Guenther K.,
Kofler Reinhard,
Huber Lukas A.
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000400
Subject(s) - scaffold protein , conditional gene knockout , signal transducing adaptor protein , endosome , biology , microbiology and biotechnology , transcriptome , mapk/erk pathway , signal transduction , knockout mouse , kinase , cell growth , phosphorylation , intracellular , gene expression , gene , genetics , phenotype
Abstract Scaffold proteins regulate intracellular MAP kinase signaling by providing critical spatial and temporal specificities. We have shown previously that the scaffold protein MEK1 partner (MP1) is localized to late endosomes by the adaptor protein p14. Using conditional gene disruption of p14 in livers of mice (p14 Δhep ) we analyzed protein and transcript signatures in tissue samples. Further biological network analysis predicted that the differentially expressed transcripts and proteins are involved in cell cycle progression and regulation of cellular proliferation. Although some of the here identified signatures were previously linked to phospho‐ERK activity, most of them were novel targets of the late endosomal p14/MP1/MEK/ERK signaling module. Finally, the proliferation defect was confirmed in a chemically induced liver regeneration model in p14 Δhep knockout mice.