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Determination of in‐gel protein concentration by a ninhydrin‐based method
Author(s) -
Kang Sung Ung,
Lubec Gert
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000388
Subject(s) - ninhydrin , proteases , chromatography , chemistry , hydrolysis , digestion (alchemy) , biochemistry , amino acid , enzyme
Until now quantification of proteins in gel‐based methods relies on the amount of protein loaded onto the gel. This does not, however, represent the amount of proteins in the gel and therefore determination of proteins within the gel is mandatory. A method to quantify proteins, both hydrophilic and hydrophobic, using in‐gel digestion with proteases, subsequent acid hydrolysis and the use of the ninhydrin reaction is herein presented.