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Immunodepletion of albumin and immunoglobulin G from bovine plasma
Author(s) -
Faulkner Simon,
Elia Giuliano,
Hillard Mark,
O'Boyle Padraic,
Dunn Michael,
Morris Dermot
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000364
Subject(s) - proteome , bovine serum albumin , antibody , proteomics , albumin , immunoglobulin g , blood proteins , chemistry , serum albumin , identification (biology) , biochemistry , biology , microbiology and biotechnology , immunology , gene , botany
Abstract Current MS‐based proteomics has facilitated the identification of large numbers of proteins from complex mixtures. The bovine plasma proteome has the potential to provide a wealth of information concerning the biological state of an animal. However, during MS‐based experiments, higher abundance proteins such as albumin and immunoglobulin G (IgG) can hinder the identification of potentially important proteins that are present in much lower abundance. While a variety of readily available technologies exist for the depletion of multiple high‐abundance proteins from human, mouse and rat samples, there are few available for bovine. In this study, we report the depletion of >97% of albumin and >92% of IgG from bovine plasma.