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A novel reactive ester derivative of biotin with reduced membrane permeability for in vivo biotinylation experiments
Author(s) -
Strassberger Verena,
Trüssel Sabrina,
Fugmann Tim,
Neri Dario,
Roesli Christoph
Publication year - 2010
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000308
Subject(s) - biotinylation , biotin , in vivo , chemistry , avidin , biochemistry , membrane , biology , microbiology and biotechnology
The in vivo perfusion of rodent models of disease with biotin derivatives and the subsequent comparative proteomic analysis of healthy and diseased tissues represent a promising methodology for the identification of vascular accessible biomarkers. A novel, triply charged biotinylation reagent, NHS‐β‐Ala‐( L ‐Asp) 3 ‐biotin, was synthesized and validated in terms of its applicability for in vivo protein biotinylation. Compared to sulfo‐NHS‐LC‐biotin, NHS‐β‐Ala‐( L ‐Asp) 3 ‐biotin exhibited a reduced membrane permeability and a preferential labeling of proteins localized in compartments readily accessible in vivo from the vasculature.