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Pseudo‐neutral‐loss scan for selective detection of phosphopeptides and N ‐glycopeptides using liquid chromatography coupled with a hybrid linear ion‐trap/orbitrap mass spectrometer
Author(s) -
Hsiao HeHsuan,
Urlaub Henning
Publication year - 2010
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000290
Subject(s) - orbitrap , chemistry , chromatography , mass spectrometry , ion trap , electrospray , quadrupole ion trap , elution , glycopeptide , electrospray ionization , phosphoric acid , ion , analytical chemistry (journal) , organic chemistry , biochemistry , antibiotics
We describe a novel method, termed “pseudo‐neutral‐loss scan” MS, for selectively probing phosphopeptides and glycopeptides on a LTQ‐Orbitrap mass spectrometer. The instrument has been programmed such that an in‐source CID energy is applied to all species that eluted from LC column and were subsequently ionised in the electrospray ion source. Ion pairs that differ in the mass of a neutral loss of phosphoric acid or monosaccharide residues are automatically selected for CID MS/MS and further for multi‐stage‐activation MS3. Our method should prove useful for a highly selective and sensitive approach to identify phosphopeptides and glycopeptides from purified protein digestion without any further enrichment, and could potentially be of use in more complex mixture.