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Proteomic analysis of human gastric juice: A shotgun approach
Author(s) -
Liang Cynthia R. M. Y.,
Tan Sandra,
Tan Hwee Tong,
Lin Qingsong,
Lim Teck Kwang,
Liu Yi,
Yeoh Khay Guan,
So Jimmy,
Chung Maxey C. M.
Publication year - 2010
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000278
Subject(s) - shotgun proteomics , proteome , saliva , stomach , proteomics , shotgun , pancreatic juice , gastric fluid , gastritis , biological fluids , mucin , chemistry , medicine , gastroenterology , biology , bioinformatics , chromatography , biochemistry , pancreas , gene
Gastric juice is the most proximal fluid surrounding the stomach tissue. The analysis of gastric juice protein contents will thus be able to accurately reflect the pathophysiology of the stomach. This biological fluid is also a potential reservoir of secreted biomarkers in higher concentration as compared to the serum. Unlike the rest of the gastrointestinal fluids, there were very few studies reported on gastric juice proteome. To date, the proteins that routinely populate this biofluid are largely unknown. This is partly due to the technical difficulties in processing a sample that contains a collection of other gastrointestinal fluids, especially saliva. In this study, we attempt to profile the protein components of the gastric fluids from chronic gastritis patients using a direct shotgun proteomics approach. These data represent the first report of the proteome of human gastric juice with gastritis background.