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A method to prevent cross contamination during 2‐DE by β‐amyloid peptides
Author(s) -
Schieb Heinke,
Spitzer Philipp,
Riess Verena,
Wiltfang Jens,
Klafki Hans W.
Publication year - 2010
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000227
Subject(s) - adsorption , contamination , aluminium oxide , peptide , chemistry , human decontamination , amyloid (mycology) , aluminum oxide , ceramic , aluminium , oxide , chromatography , biochemistry , materials science , biology , metallurgy , inorganic chemistry , organic chemistry , waste management , ecology , engineering
Abstract A method for the efficient decontamination of aluminium oxide ceramic 2‐DE focusing trays from β‐amyloid peptides (Aβ) is reported. As these contaminations were resistant to the standard cleaning procedures, additional harsh cleaning steps were necessary for their efficient removal. Our observations suggest that specific surface properties affect the degree of adsorption of the Aβ‐peptides. “Surface catalysed amyloid aggregation” in the aluminium oxide ceramic trays is proposed as a possible underlying mechanism for the occurrence of proteinase K‐resistant forms of Aβ.