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The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate‐washed membrane standard
Author(s) -
Peng Lifeng,
Kapp Eugene A.,
Fenyö David,
Kwon MinSeok,
Jiang Pu,
Wu Songfeng,
Jiang Ying,
Aguilar MarieIsabel,
Ahmed Nikhat,
Baker Mark S.,
Cai Zongwei,
Chen YuJu,
Van Chi Phan,
Chung Maxey C. M.,
He Fuchu,
Len Alice C. L.,
Liao PaoChi,
Nakamura Kazuyuki,
Ngai Sai Ming,
Paik YoungKi,
Pan TaiLong,
Poon Terence C. W.,
Hosseini Salekdeh Ghasem,
Simpson Richard J.,
Sirdeshmukh Ravi,
Srisomsap Chantragan,
Svasti Jisnuson,
Tyan YuChang,
Dreyer Florian S.,
McLauchlan Danyl,
Rawson Pisana,
Jordan T. William
Publication year - 2010
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000126
Subject(s) - proteome , proteomics , endoplasmic reticulum , membrane protein , organelle , membrane , human proteome project , integral membrane protein , chemistry , biology , computational biology , biochemistry , gene
The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse liver that contains endoplasmic reticulum and other organelles. In this study, we present the strategy used for the preparation and initial characterization of the membrane sample, including validation that the carbonate‐washing step enriches for integral and lipid‐anchored membrane proteins. Analysis of 17 independent data sets from five types of proteomic workflows is in progress.