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Two‐dimensional gel proteome reference map of INS‐1E cells
Author(s) -
D'Hertog Wannes,
Maris Michael,
Thorrez Lieven,
Waelkens Etienne,
Overbergh Lut,
Mathieu Chantal
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201000006
Subject(s) - proteome , computational biology , proteomics , biology , bioinformatics , biochemistry , gene
The insulin‐producing INS‐1E rat cell line is widely used as a model for studying β‐cells. It is a well‐characterized cell line, mainly used in diabetes research. We established a 2‐DE reference map for INS‐1E cells. Using MALDI‐TOF/TOF‐MS/MS, we identified 546 spots. These included various proteins with an important role in β‐cell physiology and with known roles as crucial proteins for diabetes development. We believe that the availability of this reference map will enhance our knowledge of β‐cell physiology.