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Strategy for comprehensive identification of human N ‐myristoylated proteins using an insect cell‐free protein synthesis system
Author(s) -
Suzuki Takashi,
Moriya Koko,
Nagatoshi Kei,
Ota Yoshinobu,
Ezure Toru,
Ando Eiji,
Tsunasawa Susumu,
Utsumi Toshihiko
Publication year - 2010
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200900783
Subject(s) - complementary dna , myristoylation , biology , fusion protein , peptide sequence , biochemistry , amino acid , microbiology and biotechnology , gene , recombinant dna , phosphorylation
To establish a strategy for the comprehensive identification of human N ‐myristoylated proteins, the susceptibility of human cDNA clones to protein N ‐myristoylation was evaluated by metabolic labeling and MS analyses of proteins expressed in an insect cell‐free protein synthesis system. One‐hundred‐and‐forty‐one cDNA clones with N ‐terminal Met‐Gly motifs were selected as potential candidates from ∼2000 Kazusa ORFeome project human cDNA clones, and their susceptibility to protein N ‐myristoylation was evaluated using fusion proteins, in which the N ‐terminal ten amino acid residues were fused to an epitope‐tagged model protein. As a result, the products of 29 out of 141 cDNA clones were found to be effectively N ‐myristoylated. The metabolic labeling experiments both in an insect cell‐free protein synthesis system and in the transfected COS‐1 cells using full‐length cDNA revealed that 27 out of 29 proteins were in fact N ‐myristoylated. Database searches with these 27 cDNA clones revealed that 18 out of 27 proteins are novel N ‐myristoylated proteins that have not been reported previously to be N ‐myristoylated, indicating that this strategy is useful for the comprehensive identification of human N ‐myristoylated proteins from human cDNA resources.