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A ubiquitin and ubiquitin‐like protein spectral library
Author(s) -
Srikumar Tharan,
Jeram Stanley M.,
Lam Henry,
Raught Brian
Publication year - 2010
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200900627
Subject(s) - ubiquitin , computational biology , proteomics , chemistry , microbiology and biotechnology , biology , biochemistry , gene
We have developed and validated a ubiquitin (Ub) and ubiquitin‐like protein (Ubl) spectral library, consisting of 467 consensus spectra (320 unique peptides derived from autophagy‐related protein 8, F‐adjacent transcript 10, interferon‐stimulated gene 15 kDa protein, neural precursor cell expressed developmentally down‐regulated protein 8, small ubiquitin‐related modifiers 1–3 and Ub, and nine of the most commonly observed Ub/Ubl chain linkages). The use of the Ub/Ubl library with a spectral matching tool (SpectraST) yields improved performance over database search engines, and can successfully identify many types of Ub/Ubl chain‐derived peptides that cannot be identified by standard database search algorithms.