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Protein subcellular localization profiling of breast cancer cells by dissociable antibody microarray staining
Author(s) -
Fu Guanyuan,
Song X. Cynthia,
Yang Xufen,
Peng Tao,
Wang Yingjian,
Zhou G. Wayne
Publication year - 2010
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200900585
Subject(s) - subcellular localization , breast cancer , antibody , profiling (computer programming) , biology , proteomics , microarray , antibody microarray , tissue microarray , microarray analysis techniques , staining , protein subcellular localization prediction , computational biology , cancer research , immunohistochemistry , cancer , gene , immunology , biochemistry , gene expression , genetics , computer science , operating system
We have developed dissociable antibody microarray (DAMA) staining technology that provides a new approach to the global analysis of protein subcellular localization (SCL) in fixed cells. We have developed and optimized this technology for protein SCL profiling, generated ChipView, a program for management and analysis of molecular image database, and utilized the technique to identify proteins with unique SCL in breast cancer cell lines. We compared the SCL profiles of 325 proteins among nine different breast cell lines, and have identified one protein, Cyclin B1, with distinctively different SCLs between normal and cancer cell lines. With classic individual immunostaining, Cyclin B1 was confirmed to localize to the cytoplasm of seven breast cancer cell lines and in both cytoplasm and nuclei of two normal breast cell lines, and to have higher expression levels in the cancer cell lines tested.