Premium
The effect of interfering ions on search algorithm performance for electron‐transfer dissociation data
Author(s) -
Good David M.,
Wenger Craig D.,
Coon Joshua J.
Publication year - 2009
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200900570
Subject(s) - electron transfer dissociation , mascot , dissociation (chemistry) , mass spectrometry , database search engine , ion , computer science , search algorithm , chemistry , algorithm , search engine , tandem mass spectrometry , information retrieval , chromatography , organic chemistry , political science , law
Collision‐activated dissociation and electron‐transfer dissociation (ETD) each produce spectra containing unique features. Though several database search algorithms ( e.g. SEQUEST, MASCOT, and Open Mass Spectrometry Search Algorithm) have been modified to search ETD data, this consists chiefly of the ability to search for c ‐ and z • ‐ions; additional ETD‐specific features are often unaccounted for and may hinder identification. Removal of these features via spectral processing increased total search sensitivity by ∼20% for both human and yeast data sets; unique peptide identifications increased by ∼17% for the yeast data sets and ∼16% for the human data set.