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A new generation of cross‐linkers for selective detection by MALDI MS
Author(s) -
Paramelle David,
Cantel Sonia,
Enjalbal Christine,
Amblard Muriel,
Forest Eric,
Heymann Michaël,
Geourjon Christophe,
Martinez Jean,
Subra Gilles
Publication year - 2009
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200900562
Subject(s) - chemistry , moiety , matrix assisted laser desorption/ionization , linker , mass spectrometry , chromatography , combinatorial chemistry , matrix (chemical analysis) , stereochemistry , organic chemistry , desorption , computer science , operating system , adsorption
We designed a new cross‐linker bearing a CHCA moiety. The use of the CHCA‐tagged cross‐linker JMV 3378 in conjunction with a neutral MALDI matrix α‐cyano‐4‐hydroxycinnamic methyl ester enabled specific signal enhancement in MALDI‐TOF MS of cross‐link containing peptides. Discrimination between modified and non‐modified peptides can be achieved by comparison of two spectra, one using CHCA and the other using the α‐cyano‐4‐hydroxycinnamic methyl ester matrix. The methodology was validated using cytochrome c and apo‐myoglobine as model proteins.