Premium
Improved reproducibility of reverse‐phase protein microarrays using array microenvironment normalization
Author(s) -
Anderson Troy,
Wulfkuhle Julia,
Liotta Lance,
Winslow Raimond L.,
Petricoin Emanuel
Publication year - 2009
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200900505
Subject(s) - normalization (sociology) , reproducibility , protein microarray , computer science , statistical power , dna microarray , computational biology , biology , chromatography , chemistry , mathematics , statistics , gene expression , genetics , sociology , anthropology , gene
We introduce a novel experimental methodology for the reverse‐phase protein microarray platform which reduces the typical measurement CV as much as 70%. The methodology, referred to as array microenvironment normalization, increases the statistical power of the platform. In the experiment, it enabled the detection of a 1.1‐fold shift in prostate specific antigen concentration using approximately six technical replicates rather than the 37 replicates previously required. The improved reproducibility and statistical power should facilitate clinical implementation of the platform.