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2‐D DIGE reveals changes in wheat xylanase inhibitor protein families due to Fusarium graminearum Δ Tri5 infection and grain development
Author(s) -
Dornez Emmie,
Croes Evi,
Gebruers Kurt,
Carpentier Sebastien,
Swennen Rony,
Laukens Kris,
Witters Erwin,
Urban Martin,
Delcour Jan A.,
Courtin Christophe M.
Publication year - 2010
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200900493
Subject(s) - xylanase , fusarium , pyrenophora , biology , microbiology and biotechnology , glume , inoculation , anthesis , population , food science , biochemistry , botany , enzyme , horticulture , gene , cultivar , medicine , environmental health
Wheat contains three different classes of proteinaceous xylanase inhibitors (XIs), i.e. Triticum aestivum xylanase inhibitors (TAXIs) xylanase‐inhibiting proteins (XIPs), and thaumatin‐like xylanase inhibitors (TLXIs) which are believed to act as a defensive barrier against phytopathogenic attack. In the absence of relevant data in wheat kernels, we here examined the response of the different members of the XI protein population to infection with a Δ Tri5 mutant of Fusarium graminearum , the wild type of which is one of the most important wheat ear pathogens, in early developing wheat grain. Wheat ears were inoculated at anthesis, analyzed using 2‐D DIGE and multivariate analysis at 5, 15, and 25 days post anthesis (DPA), and compared with control samples. Distinct abundance patterns could be distinguished for different XI forms in response to infection with F. graminearum Δ Tri5 . Some (iso)forms were up‐regulated, whereas others were down‐regulated. This pathogen‐specific regulation of proteins was mostly visible at five DPA and levelled off in the samples situated further from the inoculation point. Furthermore, it was shown that most identified TAXI‐ and XIP‐type XI (iso)forms significantly increased in abundance from the milky (15 DPA) to the soft dough stages (25 DPA) on a per kernel basis, although the extent of increase differed greatly. Non‐glycosylated XIP forms increased more strongly than their glycosylated counterparts.