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Proteomic analysis of germinating urediniospores of Phakopsora pachyrhizi , causal agent of Asian soybean rust
Author(s) -
Luster Douglas G.,
McMahon Michael B.,
Carter Melissa L.,
Fortis Laurie L.,
Nuñez Alberto
Publication year - 2010
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200900469
Subject(s) - phakopsora pachyrhizi , urediniospore , biology , soybean rust , obligate , spore germination , basidiomycota , rust (programming language) , germination , pathogen , spore , botany , microbiology and biotechnology , fungicide , computer science , programming language
Phakopsora pachyrhizi is an obligate pathogen that causes Asian soybean rust. Asian soybean rust has an unusually broad host range and infects by direct penetration through the leaf cuticle. In order to understand the early events in the infection process, it is important to identify and characterize proteins in P. pachyrhizi. Germination of the urediniospore is the first stage in the infection process and represents a critical life stage applicable to studies with this obligate pathogen. We have applied a 2-DE and MS approach to identify 117 proteins from the National Center of Biotechnology Information nonredundant protein database and a custom database of Basidiomycota EST sequences. Proteins with roles in primary metabolism, energy transduction, stress, cellular regulation and signaling were identified in this study. This data set is accessible at http://world-2dpage.expasy.org/repository/database=0018.