Premium
A gel‐free quantitative proteomics approach to investigate temperature adaptation of the food‐borne pathogen Cronobacter turicensis 3032
Author(s) -
Carranza Paula,
Grunau Alexander,
Schneider Thomas,
Hartmann Isabel,
Lehner Angelika,
Stephan Roger,
Gehrig Peter,
Grossmann Jonas,
Groebel Katrin,
Hoelzle Ludwig E.,
Eberl Leo,
Riedel Kathrin
Publication year - 2010
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200900460
Subject(s) - heat shock protein , heat shock , proteomics , chemistry , pathogen , microbiology and biotechnology , biochemistry , cronobacter , biology , gene , escherichia coli , enterobacter
Abstract The opportunistic food‐borne pathogen Cronobacter sp. causes rare but significant illness in neonates and is capable to grow at a remarkably wide range of temperatures from 5.5 to 47°C. A gel‐free quantitative proteomics approach was employed to investigate the molecular basis of the Cronobacter sp. adaptation to heat and cold‐stress. To this end the model strain Cronobacter turicensis 3032 was grown at 25, 37, 44, and 47°C, and whole‐cell and secreted proteins were iTRAQ‐labelled and identified/quantified by 2‐D‐LC‐MALDI‐TOF/TOF‐MS. While 44°C caused only minor changes in C. turicensis growth rate and protein profile, 47°C affected the expression of about 20% of all 891 identified proteins and resulted in a reduced growth rate and rendered the strain non‐motile and filamentous. Among the heat‐induced proteins were heat shock factors, transcriptional and translational proteins, whereas proteins affecting cellular morphology, proteins involved in motility, central metabolism and energy production were down‐regulated. Notably, numerous potential virulence factors were found to be up‐regulated at higher temperatures, suggesting an elevated pathogenic potential of Cronobacter sp. under these growth conditions. Significant alterations in the protein expression profile and growth rate of C. turicensis exposed to 25°C indicate that at this temperature the organism is cold‐stressed. Up‐regulated gene products comprised cold‐shock, DNA‐binding and ribosomal proteins, factors that support protein folding and proteins opposing cold‐induced decrease in membrane fluidity, whereas down‐regulated proteins were mainly involved in central metabolism.