Premium
De novo sequencing of peptides by MS/MS
Author(s) -
Seidler Joerg,
Zinn Nico,
Boehm Martin E.,
Lehmann Wolf D.
Publication year - 2010
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200900459
Subject(s) - electron transfer dissociation , peptide , dna sequencing , computational biology , chemistry , mass spectrometry , peptide sequence , biology , combinatorial chemistry , tandem mass spectrometry , chromatography , biochemistry , gene
The current status of de novo sequencing of peptides by MS/MS is reviewed with focus on collision cell MS/MS spectra. The relation between peptide structure and observed fragment ion series is discussed and the exhaustive extraction of sequence information from CID spectra of protonated peptide ions is described. The partial redundancy of the extracted sequence information and a high mass accuracy are recognized as key parameters for dependable de novo sequencing by MS. In addition, the benefits of special techniques enhancing the generation of long uninterrupted fragment ion series for de novo peptide sequencing are highlighted. Among these are terminal 18 O labeling, MS n of sodiated peptide ions, N‐terminal derivatization, the use of special proteases, and time‐delayed fragmentation. The emerging electron transfer dissociation technique and the recent progress of MALDI techniques for intact protein sequencing are covered. Finally, the integration of bioinformatic tools into peptide de novo sequencing is demonstrated.