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Can ultrasonic energy efficiently speed 18 O‐labeling of proteins?
Author(s) -
Carreira Ricardo J.,
Lodeiro Carlos,
Diniz Mario S.,
Moura Isabel,
Capelo José L.
Publication year - 2009
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200900197
Subject(s) - ultrasonic sensor , decoupling (probability) , chemistry , denaturation (fissile materials) , robustness (evolution) , analytical chemistry (journal) , biological system , chromatography , materials science , acoustics , physics , biochemistry , nuclear chemistry , biology , engineering , control engineering , gene
Abstract We report in this work on the robustness of ultrasonic energy as a tool to speed the isotopic labeling of proteins using the 18 O‐decoupling procedure. The first part of the decoupling procedure, comprising protein denaturation, reduction, alkylation and digestion, is done in 8 min under the effects of an ultrasonic field whilst the second part, the isotopic labeling, was assayed with and without the use of ultrasonic energy. Our results clearly demonstrate that the 18 O‐isotopic labeling in a decoupling procedure cannot be accelerated using an ultrasonic field.