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An optimized procedure for the capture, fractionation and proteomic analysis of proteins using hydrogel nanoparticles
Author(s) -
Rainczuk Adam,
Meehan Katie,
Steer David L.,
Stanton Peter G.,
Robertson David M.,
Stephens Andrew N.
Publication year - 2009
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200900187
Subject(s) - fractionation , proteomics , nanoparticle , chemistry , chromatography , proteome , computational biology , nanotechnology , biology , materials science , biochemistry , gene
We have developed an optimized procedure using dual size exclusion/affinity hydrogel nanoparticles to capture and comparatively analyze low molecular mass proteins directly from biological samples. The method described facilitates charge‐ and size‐dependent protein binding, direct analysis by MS or other means and is highly reproducible. A comparative analysis of the low molecular mass proteome of plasma following freeze–thaw immediately after venipuncture is used to illustrate proof‐of‐concept. The technique described is rapid and may be easily reproduced in any laboratory.