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Upc2p‐associated differential protein expression in Candida albicans
Author(s) -
Hoehamer Christopher F.,
Cummings Edwin D.,
Hilliard George M.,
Morschhäuser Joachim,
David Rogers Phillip
Publication year - 2009
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200900176
Subject(s) - candida albicans , fluconazole , corpus albicans , microbiology and biotechnology , biology , mutation , strain (injury) , fungal protein , mutant , genetics , gene , antifungal , anatomy
The gain‐of‐function mutation G648D in UPC2 causes ERG11 up‐regulation and increased fluconazole resistance in Candida albicans. In this study, we performed 2‐DE and PMF to identify proteomic alterations in an ERG11 ‐overexpressing fluconazole‐resistant C. albicans clinical isolate compared with its fluconazole‐susceptible parent strain. We identified 23 differentially expressed proteins, and among them, seven became differentially expressed in a C. albicans wild‐type strain after the introduction of a UPC2 allele carrying this mutation. These Upc2p‐regulated proteins may contribute to fluconazole resistance in C. albicans .