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Human plasma carboxylesterase 1, a novel serologic biomarker candidate for hepatocellular carcinoma
Author(s) -
Na Keun,
Lee EunYoung,
Lee HyoungJoo,
Kim KwangYoul,
Lee Hanna,
Jeong SeulKi,
Jeong AnSung,
Cho Sang Yun,
Kim Sun A.,
Song Si Young,
Kim Kyung Sik,
Cho Sung Won,
Kim Hoguen,
Paik YoungKi
Publication year - 2009
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200900105
Subject(s) - hepatocellular carcinoma , biomarker , western blot , biology , serology , glycoprotein , blot , microbiology and biotechnology , antibody , cancer research , pathology , medicine , immunology , biochemistry , gene
To identify and characterize a serologic glycoprotein biomarker for hepatocellular carcinoma (HCC), multi‐lectin affinity chromatography was used to isolate intracellular N‐linked glycoprotein fractions from five paired non‐tumor and tumor tissues. From the series of 2‐D DIGE targeted differentially expressed N‐linked glycoproteins, we identified human liver carboxylesterase 1 (hCE1), which was remarkably down‐regulated in tumor tissues, a finding confirmed by Western blot, a quantitative real‐time RT‐PCR, and immunohistochemical staining of non‐tumor and tumor tissues from total 58 HCC patients. To investigate whether hCE1 is also present in human plasma, we employed a magnetic bead‐based immunoprecipitation followed by nano‐LC‐MS/MS analysis, and we found for the first time that hCE1 is present in human plasma as opposed to that in liver tissues. That is, from normalization of hCE1 signal by the immunoprecipitation and Western blot analysis, hCE1 levels were increased in plasma specimens from HCC patients than in plasma from other disease patient groups ( e.g . liver cirrhosis, chronic hepatitis, cholangiocarcinoma, stomach cancer, and pancreatic cancer). From the receiver operating characteristic analysis in HCC, both sensitivity and specificity were shown to be greater than 70.0 and 85.0%, respectively. Thus, the high‐resolution proteomic approach demonstrates that hCE1 is a good candidate for further validation as a serologic glycoprotein biomarker for HCC.