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Accurate quantitation of standard peptides used for quantitative proteomics
Author(s) -
Bordeerat Narisa K.,
Georgieva Nadia I.,
Klapper David G.,
Collins Leonard B.,
Cross Tyra J.,
Borchers Christoph H.,
Swenberg James A.,
Boysen Gunnar
Publication year - 2009
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200900043
Subject(s) - proteomics , chromatography , quantitative proteomics , peptide , chemistry , computer science , biochemistry , gene
MS‐based proteomics has become an indispensable tool in system biology generating a need for accurate and precise quantitation of peptide standards. The presented method utilizes ultra performance LC‐MS/MS (UPLC‐MS/MS) to accurately quantify peptide standards at concentrations of 0.1–10 μM. The ability for accurate quantitation of micro‐molar concentrations has the advantages that quantitation can be performed routinely with high precision and the high sensitivity of the method minimizes the amounts required.