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Proteomics‐based identification of two novel direct targets of hypoxia‐inducible factor‐1 and their potential roles in migration/invasion of cancer cells
Author(s) -
Liao ShiHua,
Zhao XuYun,
Han YuHui,
Zhang Jing,
Wang LiShun,
Xia Li,
Zhao KeWen,
Zheng Ying,
Guo Meng,
Chen GuoQiang
Publication year - 2009
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200800922
Subject(s) - proteomics , identification (biology) , biology , hypoxia inducible factors , hypoxia (environmental) , computational biology , cancer cell , cancer , microbiology and biotechnology , cancer research , chemistry , biochemistry , gene , genetics , ecology , organic chemistry , oxygen
Hypoxia‐inducible factor‐1 (HIF‐1), consisting of oxygen‐sensitive HIF‐1α and constitutively expressed HIF‐1β subunits, is a master transcriptional activator for cellular response to hypoxia. To explore direct HIF‐1 targets, here we used differential gel electrophoresis (DIGE) to compare the HIF‐1‐regulated proteins between leukemic U937T‐cell line with and without conditional induction of HIF‐1α protein by tetracycline‐off system. Among the upregulated proteins identified, mRNA levels of annexin A1, macrophage‐capping protein (CapG), S100 calcium‐binding protein A4 (S100A4), S100A11, acyl‐CoA‐binding protein and calcyclin‐binding protein also increased. The expressions of the annexin A1, CapG and S100A4 genes were significantly induced by hypoxia in five adherent cell lines tested besides U937 cells, while their expressions were blocked by the short hairpin RNA specifically against HIF‐1α. Further luciferase reporter assay and chromatin immunoprecipitation showed that HIF‐1α directly bound to three hypoxia‐responsive elements located at intron 1 of S100A4 gene and hypoxia‐responsive element at −350 to −346 of CapG gene, which are essential for HIF‐1‐induced expression. Additionally, the role of S100A4 expression in migration and invasion of cancer cells were also confirmed. These findings would provide new sights for understanding the molecular mechanisms underlying HIF‐1 action.