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Proteomic analysis of chicory root identifies proteins typically involved in cold acclimation
Author(s) -
Degand Hervé,
Faber AnneMarie,
Dauchot Nicolas,
Mingeot Dominique,
Watillon Bernard,
Cutsem Pierre Van,
Morsomme Pierre,
Boutry and Marc
Publication year - 2009
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200800744
Subject(s) - cichorium , spots , inulin , proteomics , proteome , fructose , abiotic stress , biochemistry , chemistry , heat shock protein , proteolysis , trypsin , food science , biology , botany , enzyme , gene
Chicory ( Cichorium intybus ) roots contain high amounts of inulin, a fructose polymer used as a storage carbohydrate by the plant and as a human dietary and prebiotic compound. We performed 2‐D electrophoretic analysis of proteins from root material before the first freezing period. The proteins were digested with trypsin and the peptides analyzed by MS (MALDI‐TOF/TOF). From the 881 protein spots analyzed, 714 proteins corresponded to a database accession, 619 of which were classified into functional categories. Besides expected proteins ( e.g . related to metabolism, energy, protein synthesis, or cell structure), other well‐represented categories were proteins related to folding and stability (49 spots), proteolysis (49 spots), and the stress response (67 spots). The importance of abiotic stress response was confirmed by the observation that 7 of the 21 most intense protein spots are known to be involved in cold acclimation. These results suggest a major effect of the low temperature period that preceded root harvesting.