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Identification and comparative analysis of the structural proteomes of ϕKZ and EL, two giant Pseudomonas aeruginosa bacteriophages
Author(s) -
Lecoutere Elke,
Ceyssens PieterJan,
Miroshnikov Konstantin A.,
Mesyanzhinov Vadim V.,
Krylov Victor N.,
Noben JeanPaul,
Robben Johan,
Hertveldt Kirsten,
Volckaert Guido,
Lavigne Rob
Publication year - 2009
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200800727
Subject(s) - capsid , proteome , bacteriophage , biology , pseudomonas chlororaphis , pseudomonas aeruginosa , computational biology , genetics , proteomics , gene , mutant , pseudomonas , microbiology and biotechnology , bacteria , escherichia coli
Giant bacteriophages ϕKZ and EL of Pseudomonas aeruginosa contain 62 and 64 structural proteins, respectively, identified by ESI‐MS/MS on total virion particle proteins. These identifications verify gene predictions and delineate the genomic regions dedicated to phage assembly and capsid formation (30 proteins were identified from a tailless ϕKZ mutant). These data form the basis for future structural studies and provide insights into the relatedness of these large phages. The ϕKZ structural proteome strongly correlates to that of Pseudomonas chlororaphis bacteriophage 201ϕ2‐1. Phage EL is more distantly related, shown by its 26 non‐conserved structural proteins and the presence of genomic inversions.