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Two‐dimensional proteome reference map for the radiation‐resistant bacterium Deinococcus geothermalis
Author(s) -
Liedert Christina,
Bernhardt Jörg,
Albrecht Dirk,
Voigt Birgit,
Hecker Michael,
SalkinojaSalonen Mirja,
Neubauer Peter
Publication year - 2010
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200800657
Subject(s) - proteome , deinococcus , biology , proteomics , deinococcus radiodurans , enzyme , function (biology) , bacteria , biochemistry , microbiology and biotechnology , genetics , gene
2‐DE reference maps for Deinococcus geothermalis cytosolic and cell envelope proteomes were constructed. In total, 403 spots were identified as 299 different proteins. Unique in the proteomes were four subunits of V‐type ATPase and Deinococcus specific proteins constituting one‐fourth of cell envelope proteome. The cytoplasmic proteome included enzymes of the central carbon metabolism, chaperones, enzymes of protein and DNA repair, and oxidative stress. A total of 34 abundant proteins with unknown function may relate to the extreme stress tolerance of D. geothermalis .