Premium
Identification of proteins of Neisseria meningitidis induced under iron‐limiting conditions using the isobaric tandem mass tag (TMT) labeling approach
Author(s) -
van Ulsen Peter,
Kuhn Karsten,
Prinz Thorsten,
Legner Harald,
Schmid Peter,
Baumann Christian,
Tommassen Jan
Publication year - 2009
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200800642
Subject(s) - tandem mass tag , isobaric labeling , neisseria meningitidis , isobaric process , orfs , quantitative proteomics , tandem mass spectrometry , chemistry , biology , proteomics , biochemistry , gene , computational biology , genetics , chromatography , mass spectrometry , bacteria , peptide sequence , physics , open reading frame , thermodynamics
Abstract Isobaric labeling reagents such as Tandem Mass Tags (TMT®) enable the genome‐wide quantification of protein expression levels under different conditions using a gel‐free MS/MS‐based approach. Here, we applied a TMTduplex approach with two isobaric tags to study the response of the human pathogen Neisseria meningitidis to deprivation of iron, a condition met in the human body. In total, 609 proteins were identified in samples of three independent growth experiments, in which we compared cultures grown in the presence and absence of iron. Expression of 35 proteins was found to be induced or repressed under iron‐limiting conditions, including 11 proteins whose ORFs were not previously identified in DNA array studies as being regulated by iron availability at the transcriptional level. These 11 proteins include proteins likely involved in iron metabolism.