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Proteomic analysis of a filamentous fungal endophyte using EST datasets
Author(s) -
Bassett Shalome A.,
Bond Judy J.,
Kwan Fiona Y. S.,
McCulloch Alan F.,
Haynes Paul A.,
Johnson Richard D.,
Bryan Gregory T.,
Jordan T. William
Publication year - 2009
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200800585
Subject(s) - biology , endophyte , computational biology , proteomics , eukaryote , ascomycota , fungal protein , genome , botany , genetics , peptide sequence , gene
Proteomic analysis of many species of fungi, particularly filamentous fungi, is difficult due to the lack of publicly available genome sequence data and the problems associated with cross‐species comparisons. Furthermore, the detection of fungal proteins in biological systems where there are a greater number of proteins present from other eukaryote species provides additional challenges. We present an EST‐based approach for identifying proteins from a fungal endophyte of temperate grasses and demonstrate that this method is well suited for fungi with minimal sequence data.